Elderberry Minimizes and Inhibits Flu Virus Propagation

A recent study by a group of Chemical and Biomlolecular Engineering researchers from the University of Sydney’s Faculty of Engineering and IT has determined exactly how a popular ancient remedy, the elderberry fruit, can help the fight against influenza.

Conducted by Professor Fariba Deghani, Dr Golnoosh Torabian and Dr Peter Valtchev as part of the ARC Training Centre for the Australian Food Processing Industry that was established in the Faculty of Engineering and IT, the study showed that compounds from elderberries can directly inhibit the virus’s entry and replication in human cells, and can help strengthen a person’s immune response to the virus.

Although elderberry’s flu-fighting properties have long been observed, the group performed a comprehensive examination of the mechanism by which phytochemicals from elderberries combat influenza infections.

“What our study has shown is that the common elderberry has a potent direct antiviral effect against the flu virus,” said Dr Golnoosh Torabian. “It inhibits the early stages of an infection by blocking key viral proteins responsible for both the viral attachment and entry into the host cells.”

The researchers used commercially farmed elderberries which were turned into a juice serum and were applied to cells before, during and after they had been infected with the influenza virus.

The phytochemicals from the elderberry juice were shown to be effective at stopping the virus infecting the cells, however to the surprise of the researchers they were even more effective at inhibiting viral propagation at later stages of the influenza cycle when the cells had already been infected with the virus.

Elderberry showed mild inhibitory effect at the early stages of the influenza virus cycle, with considerably stronger effect (therapeutic index of 12 ± 1.3) in the post-infection phase. The data further support both direct effects of elderberry extract by blocking viral glycoproteins as well as indirect effects by increased expression of IL-6, IL-8, and TNF. Cyn 3-glu despite demonstrating a similar direct mechanism of action (IC50 of 0.069 mg/ml) compared to the elderberry juice, did not affect the expression of pro-inflammatory cytokines.

“This observation was quite surprising and rather significant because blocking the viral cycle at several stages has a higher chance of inhibiting the viral infection,” explained Dr Peter Valtchev.

“In addition to that, we identified that the elderberry solution also stimulated the cells to release certain cytokines, which are chemical messengers that the immune system uses for communication between different cell types to coordinate a more efficient response against the invading pathogen,” said Centre Director, Professor Fariba Deghani. The team also found that the elderberry’s antiviral activity can be attributed to its anthocyanidin compounds — phytonutrients responsible for giving the fruit its vivid purple coloring.

In conclusion, the researchers said elderberry exhibits multiple modes of therapeutic action against influenza infection.

 

Source: Golnoosh Torabian, Peter Valtchev, Qayyum Adil, Fariba Dehghani. Anti-influenza activity of elderberry (Sambucus nigra). Journal of Functional Foods, 2019; 54: 353 DOI: 10.1016/j.jff.2019.01.031