Microalgae Chlamydomonas May Improve Gastrointestinal Health

In this recent study, researchers explored the benefits of a single cell algae for gastrointestinal health. For decades, the green algae, Chlamydomonas reinhardtii, which primarily grows in wet soil, has served as a model species for research topics spanning from algae-based biofuels to plant evolution. While other species of algae have been used as dietary nutraceuticals that provide beneficial oils, vitamins, proteins, carbohydrates, antioxidants and fiber, the benefits of consuming C. reinhardtii were previously unexplored.

algae for gastrointestinal health

Researchers at the University of California San Diego recently completed the first study examining the effects of consuming C. reinhardtii and demonstrated that the algae improves human gastrointestinal issues associated with irritable bowel syndrome (IBS) such as diarrhea, gas and bloating. Results of the project are published in the Journal of Functional Foods.

“People have been looking at this algae for decades, but this is the first study to show what many of us have suspected–it’s good for you,” said principal investigator and algae expert Stephen Mayfield, a distinguished professor in UC San Diego’s Division of Biological Sciences and co-director of the Food and Fuel for the 21st Century Program (FF21). “This is exciting because it demonstrates a clear benefit: If you have IBS-like symptoms, this is good for you.”

For years researchers in Mayfield’s laboratory have been exploring C. reinhardtii as a cost-competitive and sustainable source of valuable plant-based products, specifically pharmaceuticals and biofuels. Now, working with several collaborators, including UC San Diego’s John Chang (School of Medicine), Rob Knight (School of Medicine, Jacobs School of Engineering and Center for Microbiome Innovation) and the San Diego-based startup Triton Algae Innovations, they turned their attention towards investigating the algae as a nutritious food additive for improving human health.

The C. reinhardtii biomass used in the study, which was grown by Triton Algae Innovations, was subject to rigorous safety testing and designated as “Generally Recognized As Safe” by the U.S. Food and Drug Administration, green-lighting the use of the organism in a human study.

Preliminary data in mouse studies demonstrated that consuming C. reinhardtii significantly reduced the rate of weight loss in mice with acute colitis, which is generally linked to inflammation of the digestive tract. Building off these results, the researchers set out to test for a similar effect when the algae was consumed by human volunteers, including those with and without symptoms associated with IBS. Volunteers consumed daily spoonfuls of powdered C. reinhardtii biomass and reported their gastrointestinal health for one month. Of the hundreds of interested participants in the project, data from 51 volunteers met the study’s requirements for inclusion in the final data analyses.

Results showed that participants who suffered from a history of frequent gastrointestinal symptoms reported significantly less bowel discomfort and diarrhea, significantly less gas or bloating and more regular bowel movements.

“The benefits of consuming this species of algae were immediately obvious when examining the data from both mice and humans who suffered from gastrointestinal symptoms,” said Frank Fields, a research scientist in Mayfield’s lab and lead author of the paper. “I hope that this study helps destigmatize the thought of incorporating algae and algae-based products into your diet–it is a fantastic source of nutrition and we have now shown that this species of algae has additional benefits to animal and human health.”

Volunteers also were provided with stool sampling kits and sent samples to the American Gut Project, a citizen science effort led by Knight and his lab, to assess any changes in their microbiomes. The results indicated that the gut microbiome composition remained diverse, which is typical of healthier individuals, and that no significant changes to the composition of their gut microbiome occurred during the study as a result of consuming the algae.

The researchers say much more testing with larger groups of participants across longer time periods is needed. At this point, they are unclear about how the algae works to improve gastrointestinal health. The scientists believe the benefits could be traced to a bioactive molecule in algae or perhaps a change in gene expression of gut bacteria caused by algae consumption.

Still, the observed results in human volunteers led them to conclude in the paper that “the addition of C. reinhardtii into the diet will not only add nutritional value but may also function to relieve some gastrointestinal symptoms of certain individuals.” See below.

  • C. reinhardtii significantly mitigated weight loss in a murine model of acute colitis.
  • C. reinhardtii positively impacted gastrointestinal symptoms in humans.
  • C. reinhardtii had no adverse effect on the microbial composition of participants.
Appearance, composition, and perception of Chlamydomonas reinhardtii biomass used in this study. Participants were provided with a jar containing lyophilized C. reinhardtii biomass and a scoop that held 0.5 g of biomass (left) and asked for their opinion of the appearance, smell, and taste of the biomass (right; N = 51).

Conclusion/ “This study examined for the first time the effects of consuming whole-cell biomass of the green microalgae Chlamydomonas reinhardtii in both mice and humans, with an emphasis on gut health. It was found that in both cases the addition of C. reinhardtii biomass to the diet had a beneficial impact on gastrointestinal function. In mice, administration of C. reinhardtii to their diet significantly mitigated weight loss in a model of colitis. In humans, the addition of C. reinhardtii to the diet of individuals with high-frequency of gastrointestinal symptoms, like those commonly associated with irritable bowel syndrome, significantly reduced the occurrence of bowel discomfort, diarrhea, gas, and bloating while also increasing the regularity of bowel movements and improving the quality of stools.

Conversely, when examining the microbiomes of these human participants, consuming C. reinhardtii caused no significant change to the microbial composition of the gut. This suggests that the reported benefits are perhaps caused by a bioactive molecule in the algal biomass or a potentially a shift in gene expression of the gut bacteria brought about by consuming the algae. However, in order to solidify either of these deductions, additional research must be conducted with a greater number of volunteers in order to account for effects attributed to differences in age, gender, weight, and diet. Nevertheless, we conclude that the addition of C. reinhardtii into the diet will not only add nutritional value but may also function to relieve some gastrointestinal symptoms in certain individuals.”

Source: Frances Fields, et al. Effects of the microalgae Chlamydomonas on gastrointestinal health. J of Functional Foods. 2020; 65. https://doi.org/10.1016/j.jff.2019.103738